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PD-L2 Monoclonal Ant...

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PD-L2 Monoclonal Antibody

  恒遠生物抗體平臺現已擁有4萬多種抗體(含單抗和多抗),包括人類常用抗體,模式生物類相關抗體、小分子抗體、二抗、標簽抗體及內參抗體等。并以每年4000種的速度在持續增長。

  恒遠生物擁有專業的質控團隊,配備先進實驗儀器,現已成功地建立了ChIP平臺,因此我們的抗體不僅能提供Biotin、FITC、HRP等標記,大部分抗體都能進行ELISA、WB、IHC、IF、IP、ChIP等應用驗證。

  我們致力于為所有用戶提供高親和力、高特異性的優質抗體。

PD-L2 Monoclonal Antibody

 
  • 貨號:
    HYK-200026
  • 規格:
     
  • 圖片:
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate
      All lanes PD-L2 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 31,21 KDa
      Observed band size: 45-50 KDa
      Exposure time:5min

       

    • Western Blot
      Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg
      All lanes: PD-L2 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 31,21 KDa
      Observed band size: 45-50 KDa
      Exposure time:5min

       

    • Western Blot
      Positive WB detected in: 15μg hela whole cell lysate PD-L2 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 31,21 KDa
      Observed band size: 45-50 KDa
      Exposure time:5min

    • IHC image of CSB-MA017667A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

    • Immunofluorescence staining of HepG2 cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

    • Immunofluorescence staining of Hela cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

    • Immunofluorescence staining of Raji cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

    • Immunofluorescence staining of U251 cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

    • Overlay histogram showing 293 cells transfected with PD-L2 stained with CSB-MA017667A0m (red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2μg/1*106cells) for 1 h at 4°C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (2μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

產品詳情

  • 產品名稱:
    Mouse anti-Homo sapiens (Human) PDCD1LG2 Monoclonal Antibody antibody
  • Uniprot No.:
    Q9BQ51
  • 基因名:
    PDCD1LG2
  • 別名:
    B7 dendritic cell molecule antibody; B7-DC antibody; B7DC antibody; bA574F11.2 antibody; Btdc antibody; Butyrophilin B7 DC antibody; Butyrophilin B7-DC antibody; Butyrophilin B7DC antibody; CD 273 antibody; CD273 antibody; CD273 antigen antibody; MGC142238 antibody; MGC142240 antibody; PD 1 ligand 2 antibody; PD L2 antibody; PD-1 ligand 2 antibody; PD-L2 antibody; PD1 ligand 2 antibody; PD1L2_HUMAN antibody; PDCD 1 ligand 2 antibody; PDCD1 ligand 2 antibody; PDCD1L2 antibody; Pdcd1lg2 antibody; PDL 2 antibody; PDL2 antibody; Programmed cell death 1 ligand 2 antibody; Programmed death ligand 2 antibody
  • 宿主:
    Mouse
  • 反應種屬:
    Human
  • 免疫原:
    Recombinant Human Programmed cell death 1 ligand 2 protein (21-118AA)
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal Antibody
  • 抗體亞型:
    IgG2b
  • 純化方式:
    >95%,Protein A purified
  • 克隆號:
    7F11D11
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IHC, IF, FC, IP
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:5000-1:640000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
    IP 2μl-5μl
  • Protocols:
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
    Immunoprecipitation (IP) Protocol
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.


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